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3 μg of the luciferase reporter plasmid pgas-luc  (Lonza)
90
Lonza 3 μg of the luciferase reporter plasmid pgas-luc
NKLAM positively affects STAT1-mediated transcriptional activity. A) WT (black column) and NKLAM-KO (white column) BMDM were nucleofected with <t>pGAS-luc</t> plasmid then stimulated with 100 U/mL IFNγ for 6 h and assayed for luciferase activity; *p < 0.05; n = 5. B–C) WT (black column) and NKLAM-KO (white column) BMDM were treated with 100 U/mL IFNγ for 3 or 6 h. The expression of iNOS and CCL5 was determined by quantitative RT-PCR. The fold change in mRNA levels (mean ± SEM) is expressed relative to untreated cultures (solid line set at 1). Graph represents at least 3 independent experiments. *p < 0.05; n = 3.
3 μg Of The Luciferase Reporter Plasmid Pgas Luc, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 μg of the luciferase reporter plasmid pgas-luc/product/Lonza
Average 90 stars, based on 1 article reviews
3 μg of the luciferase reporter plasmid pgas-luc - by Bioz Stars, 2026-05
90/100 stars
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NKLAM positively affects STAT1-mediated transcriptional activity. A) WT (black column) and NKLAM-KO (white column) BMDM were nucleofected with pGAS-luc plasmid then stimulated with 100 U/mL IFNγ for 6 h and assayed for luciferase activity; *p < 0.05; n = 5. B–C) WT (black column) and NKLAM-KO (white column) BMDM were treated with 100 U/mL IFNγ for 3 or 6 h. The expression of iNOS and CCL5 was determined by quantitative RT-PCR. The fold change in mRNA levels (mean ± SEM) is expressed relative to untreated cultures (solid line set at 1). Graph represents at least 3 independent experiments. *p < 0.05; n = 3.

Journal: Cellular signalling

Article Title: E3 ubiquitin ligase NKLAM ubiquitinates STAT1 and positively regulates STAT1-mediated transcriptional activity

doi: 10.1016/j.cellsig.2016.08.014

Figure Lengend Snippet: NKLAM positively affects STAT1-mediated transcriptional activity. A) WT (black column) and NKLAM-KO (white column) BMDM were nucleofected with pGAS-luc plasmid then stimulated with 100 U/mL IFNγ for 6 h and assayed for luciferase activity; *p < 0.05; n = 5. B–C) WT (black column) and NKLAM-KO (white column) BMDM were treated with 100 U/mL IFNγ for 3 or 6 h. The expression of iNOS and CCL5 was determined by quantitative RT-PCR. The fold change in mRNA levels (mean ± SEM) is expressed relative to untreated cultures (solid line set at 1). Graph represents at least 3 independent experiments. *p < 0.05; n = 3.

Article Snippet: WT or NKLAM-KO macrophages (4 × 10 6 ) were mixed with 3 μg of the luciferase reporter plasmid pGAS-luc and suspended in nucleofection solution T (Lonza).

Techniques: Activity Assay, Plasmid Preparation, Luciferase, Expressing, Quantitative RT-PCR